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Human Campylobacter Fecal ELISA Kit

NR-R10178

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSES 

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$586.80

Data sheet

Validity 12 months from manufacture date
Background Infection by thermophilic Campylobacter species is a leading cause of human gastroenteritis. Of the various species of Campylobacter, C. jejuni, C. coli and C. lari, are the species most often associated with human illness. Campylobacter are often passed to humans through the handling or consumption of contaminated food, particularly foods of animal origin. Recently, human infection with Campylobacter has been implicated in the induction of Guillain-Barré Syndrome (GBS) and reactive arthritis. GBS is a debilitating and potentially fatal neurological disease that produces paralysis. Campylobacter species are gram negative, motile curved or spiral rods that require highly specialized growth conditions. Typical cultivation entails pre-enrichment and enrichment steps in broth, followed by isolation on a selective solid medium. Of particular importance in the cultivation of Campylobacter is the requirement for a microaerobic atmosphere.
Assay principle Campylobacter ELISA is a double antibody (sandwich) immunoassay utilizing specific antiCampylobacter antibodies coated to microwells. After addition of the sample and the enzyme conjugate, a positive reaction (indicating the presence of Campylobacter antigen) produces a deep blue color. Addition of the Stop Solution ends the assay and turns the blue color to yellow. The results may be read visually or with an ELISA reader.
INTENDED USE This Diagnostic Automation Inc. microwell enzyme linked immunoabsorbant assay (ELISA) detection kit is an in vitro diagnostic immunoassay for the detection of antigen to Campylobacter species in human feces using peroxidase as the indicator enzyme. The assay may be read visually or with an ELISA reader. This ELISA is intended to be used with stools that are fresh or frozen.
Assay Type Indirect ELISA
Kit Type Colorimetric
Sample Type 1 gm stool sample
Assay Range Qualitative Positive; Negative control
Sensitivity 77%
Specificity 100%

More info

This kit is used to qualitatively detect Campylobacter antigen in Human stool.

Key Components:


Test Strips

Microwells containing anti-Campylobacter polyclonal antibodies: 96 Test Wells. 

 

Enzyme Conjugate 

One (1) bottle containing 11 ml of anti-Campylobacter antibodies conjugated to horseradish peroxidase with Thimerosal.

 

Positive Control

One (1) vial containing 2 ml of Campylobacter antigen in a buffered base.  Negative Control One (1) vial containing 2 ml of buffered base. 

 

Negative Control

One (1) vial containing 2 ml of buffered base.

 

Chromogen

One (1) bottle containing 11 ml of the chromogen tetramethylbenzidine (TMB). 

 

Wash Concentrate (20X)

Two (2) bottles containing 25 ml of concentrated buffer and Thimerosal. 

 

Stop Solution

One (1) bottle containing 11 ml of 5% phosphoric acid solution.


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