Mesothelin  Antibody View larger

Mesothelin Antibody

ASA-B1266

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

mesothelin Polyclonal IgG Rabbit Human ELISA Immunogen affinity purified.

Immunogen

E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

MSLN

Protein

Mesothelin

Uniprot ID

Q13421

Function

Membrane-anchored forms may play a role in cellular adhesion.

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Sub-cellular localization

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.

Sequence Similarities

Aliases

CAK 1 antibody|CAK1 antibody|CAK1 antigen antibody|cleaved form antibody|Megakaryocyte potentiating factor antibody|Mesothelin antibody|Mesothelin isoform 1 precursor antibody|MPF antibody|Msln antibody|MSLN_HUMAN antibody|Pre pro megakaryocyte potentiating factor antibody|Pre-pro-megakaryocyte-potentiating factor antibody|SMR antibody|SMRP antibody|Soluble MPF mesothelin related protein antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
ELISA 0.1-0.5μg/ml HumanSandwich ELISA format

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Mesothelin antibody, ASA-B1266, Western blotting
All lanes: Anti Mesothelin (ASA-B1266) at 0.5ug/ml
WB: Recombinant Human Mesothelin Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
Anti- Mesothelin antibody, ASA-B1266,IHC(P)
IHC(P): Human Lung Cancer Tissue

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