Filaria IgG4 ELISA Kit

Filaria IgG4 ELISA Kit
Assay Range
Qualitative: Positive & Negative Control
immunoassay. Positive, negative and low positive control samples are provided to maintain the kit’s integrity. In this assay, control samples and unknown serum samples are diluted into the Filaria Sample Dilution Buffer, then i ncubated in microtiter wells which have been coated with IWb123 (1 -6), followed by incubation with anti-human IgG4 antibody labeled with the enzyme horseradish peroxidase (HRP). After the incubation and a washing step, the wells are incubated with a tetramethylbenzidine (TMB) substrate. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. The absorbance measured is directly proportional to the concentration of specific IgG4 antibodies to IWb123 present. A set of positive and negative controls are provided as internal controls. These are provided to monitor the integrity of the kit components.
Assay principle
The Filaria IgG4 ELISA consists of one enzymatic ally amplified sandwich -type
Sample type
10µl Serum

The Filaria IgG4 ELISA is for qualitative detection of specific IgG4 antibodies in specimens to highly specific antigen IWb123 target antigen expressed primarily in infective stage larvae (L3) of the lymphatic-dwelling parasite Wuchereria bancrofti (Wb). This product is for research use only. Not for use in diagnostic procedures


All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

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