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Human Proteinase anti-neutrophil cytoplasmic antibody (PR3-ANCA) ELISA Kit

NR-R10116

$552.00

More info

Principle
Indirect ELISA, Antigen coated plate
 
Detection Range
Qualitative Positive; Negative and Cut off
 
Size
96T
 
Specificity                                   95%
Sensitivity                                   98%
 
Assay time
~ 90 min
 
Shelf Life
12 Months from the Manufacturing Date
 
Storage
Store at 2 - 8ºC. 
 
Sample volume
10 µL Serum or Plasma
 
Detection Method
Chromogenic
  
Kit Components
 
 
 
1. Low Positive Control: 1vial 
 
 
2. One 96-well plate precoated with recombinant antigen
 
 
3. Assa diluent buffer: 1 vial
 
 
4. HRP conjugated: 1 vial
 
 
5. High Positive Control: 1 vial
 
 
6. Negative Control:1vial
 
 
7. Calibrator: 1 vial
 
 
8. TMB substrate: 1 vial
 
 
9. TMB stop solution: 12 ml.

 

Background

 

Proteinase 3, also known as PRTN3, is a 29 kD protein found in the primary granules of neutrophils and monocytes. PRTN3 is a serine protease with broad proteolytic activity against a variety of extracellular matrix proteins. Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Its exact role in the function of the neutrophil is unknown, but, in human neutrophils, proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease granulomatosis with polyangiitis. 

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