Human Cancer Antigen CA15-3/Muc1/KL-6 ELISA Kit View larger

Human Cancer Antigen CA15-3/Muc1/KL-6 ELISA Kit

For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction

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$735.00

Data sheet

Validity Shelf Life 6 Months from the manufacturing date
Background Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year. Roughly half of these newly diagnosed patients are node- negative; however 30% of these cases progress to metastatic disease. There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have an aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent-S phase profile, epidermal growth factor receptor, HER-2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA15-3/Muc1/KL6. CA15-3/MUC1/KL6 is most useful for monitoring patients postoperatively for recurrence, particularly metastatic diseases. 96% of patients with local and systemic recurrence have elevated CA15-3/Muc1/KL6 which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3/MUC1/KL6 is associated with progression of carcinoma. A 50% decrease in serum CA15-3/MUC1/KL6is associated with response to treatment. CA15-3/MUC1/KL6 are more sensitive than CEA in early detection of breast cancer recurrence. CA15-3/MUC1/KL6 has been shown to be useful in early detection of relapse of ovarian cancer. CA15-3/MUC1/KL6 levels are also increased in colon, lung and hepatic tumors.
Assay principle The CA15-3/MUC1/KL6ELISA test is a two-site solid phase enzyme immunoassay. The molecules of CA15-3/MUC1/KL6 are "sandwiched" between two monoclonal antibodies. One coated to the bottom of the wells of the microtiter plates and the other linked to the horseradish peroxidase (enzyme conjugate). After incubation and washing, the enzymatic reaction develops a color which is proportional to the amount of CA15-3/MUC1/KL6 molecules present in the assay.
INTENDED USE The CA15-3/Muc1/KL-6 ELISA test is intended for use as a monitoring and screening test for breast cancer. An abnormal result (i.e., elevated serum KCA15-3/Muc1/KL-6 level) indicates further clinical management. CA15-3/Muc1/KL-6 is a useful tumor marker for patients in clinical remission following treatment. Post-operative serum CA15-3/Muc1/KL-6 values which fail to return to normal, strongly suggest the presence of residual tumor, while tumor recurrence is often accompanied by a rise of serum CA15-3/Muc1/KL-6 before progressive disease is clinically evident.
Alternate Names MUC1, ADMCKD, ADMCKD1, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, mucin 1, cell surface associated
Assay Type Sandwich ELISA
Kit Type Colorimetric
Assay Range 0-240U/mL
Sensitivity 5 U/mL
Specificity 97.5%
Dilution Factors Serum sample diluted 1:50 in dilution buffer.
Gene ID 4582
UniProt ID P15941, Q7Z551

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Materials provided with the test kits

Monoclonal Anti-CA15-3/MUC1/KL6 antibody coated microtiter plate with 96 wells.  

Sample diluent, 100 ml.

Enzyme conjugate reagent, 12 ml.

CA15-3/MUC1/KL6 reference standard set, containing 0, 15, 30, 60, 120, and 240 Unit/ml (liquid, ready for use) or lyophilized form.

Wash Buffer Concentrate (50X), 15 ml.

TMB Substrate, 12 ml.

Stop solution, 12 ml.

Control set (optional)

For updated manual, please contact us at techsupport@novateinbio.com

Human Cancer Antigen CA15-3/Muc1/KL-6 ELISA Kit

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