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Human Brucella IgA ELISA Kit

NR-R10018

$1,018.80

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Principle
Sandwich Complex
 
Detection Range
Qualitative Positive; Negative; weak postive
 
Size
96T
 
Sensitivity
99%
 
Specificity
99%
 
Assay time
~ 120 min
 
Shelf Life
12 ~ 18 Months from the Manufacturing Date
 
Storage
Store at 2 - 8ºC. 
 
Sample volume
100 µL Serum or Plasma
 
Detection Method
Chromogenic
 

 

Kit Components
 
 
 
1. One 96-well plate precoated with recombinant antigen 
 
 
2. Assa diluent buffer: 12 mL x 1
 
 
3. HRP conjugated: 1 vial
 
 
4. Negative Control:1vial
 
 
5. Positive Control: 1 vial
 
 
6. 20 x Wash buffer: 1 vial
 
 
7. Cut-off control: 1 vial
 
 
8. Weak positive control: 1 vial

                                       9. TMB substrate: 12 ml
 
 
10. TMB stop solution: 12 ml

 

Background

 

Brucella is a genus of Gram-negativebacteria, which are small (0.5 to 0.7 by 0.6 to 1.5 µm), non-motile, non-encapsulatedcoccobacilli, flagellated which function as facultative intracellular parasites. Brucella is the cause of brucellosis, which is a zoonosis. It is transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. Brucellosis can affect any organ or organ system, and 90% of patients have a cyclical (undulant) fever. Though variable, symptoms can also include these clinical signs: headache, weakness, arthralgia, depression, weight loss, fatigue, and liver dysfunction. Foul smelling perspiration is considered a classical sign. About 20-60% of cases have osteoarticular complications - arthritis, spondylitis, or osteomyelitis. Brucella Coombs test and ELISA can be used to diagnose. The monitoring of antibodies can serve as a usual indication of the status of infection. The prognosis for brucellosis before the use of antibiotics had a mortality of 2%, mainly due to endocarditis. Permanent nerve deafness and spinal cord damage often occurred. 

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