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Data sheet
| Assay principle | This Chicken IFN Gamma ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Chicken IFN Gamma specific antibody has been precoated onto 96-well plate. The test samples and the biotinylated Chicken IFN Gamma specific detection antibody are added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP is added and unbound conjugates are washed away with Wash Buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic Stop Solution. The density of yellow is proportional to the Chicken IFN Gamma amount of sample captured in plate. |
| INTENDED USE | The kit is used to quantify the Chicken IFN Gamma in cell culture supernatants, serum and plasma. |
| Alternate Names | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
| Assay Type | Sandwich ELISA |
| Kit Type | ELISA Colorimetric |
| Assay Time | 2.5 hours |
| Sample Type | plasma, serum, cell culture medium |
| Assay Range | 15.6 - 1000 pg/ml |
| Sensitivity | 1.0 pg/ml |
| Specificity | This kit recognizes chicken IFN-gamma in samples. No significant cross-reactivity or interference between Chicken IFN gamma and analogues was observed. |
| Recovery | 86% - 98% |
| Precision | Intra Assay, 5.8%; Inter-Assay, 9.8%; |
| Sample Volume | 100 ul |
| Dilution Factors | Varies |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Product Citations | Available now |
| Satisfaction | 100% |
| Country of origin | The U.S. |
| Tech support | 24/7 |
| Bulk order pricing | Contact sales@novateinbio.com |
| Distributor | Worldwide |
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Citations
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1) M E Sedeik, A R Elbestawy, N A El-shall, M E Abd El-Hack, I M Saadeldin, A A Swelum, Comparative efficacy of commercial inactivated Newcastle disease virus vaccines against Newcastle disease virus genotype VII in broiler chickens, Poultry Science, Volume 98, Issue 5, May 2019, Pages 2000–2007, https://doi.org/10.3382/ps/pey559 2) Action Mechanism and Immunity Status of Broiler Chickens after Spray and Drinking Water Administration of Kanne-Brottrunk, Raheel I.A., Orabi A. , Saber El-Hanbaly, Hassan Shaheen, 3) Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea, A Fernández, et al, Pakistan Veterinary Journal, 2015, 2074-7764 4) Effect of arginine or glutamine supplementation on production, organ weights, interferon gamma, interleukin 6 and antibody titre of broilers, Szabó József et al, Acta Veterinaria Hungarica, 2014, Volume 62, Issue 3 5) Treasing on Tylvamyco® as a Novel Immunomodulatory Medication on Broiler Chickens. Bastamy M, I Raheel, H Ellakany and A Orabi, 2020. Treasing on Tylvamyco® as a Novel Immunomodulatory Medication on Broiler Chickens. Int J Vet Sci. DOI: 10.37422/IJVS/20.074 |