Human Cardiotrophin-1 ELISA Kit

Human Cardiotrophin-1 ELISA Kit
FM-E100316
$635.00


Assay Range

31.2-2,000 pg/mL  

Sensitivity

10.0 pg/mL

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Human Cardiotrophin-1 standard: 2 vials

 2. One 96-well plate coated with Human Cardiotrophin-1  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1

 

 

Background

 

Cardiotrophin1 (CT-1) is a member of the IL-6 family and is widely expressed in the heart, skeletal muscle, prostate, ovary, lung, kidney, pancreas, thymus, testis and small intestine with a higher level at heart and skeletal muscle. CT-1 is a cardiac hypertrophic factor with overlapping functions with other members of IL-6 family. Human and mouse CT-1 share 80% aa sequence identity and exhibit species cross-reactivity. CT-1 initiates its downstream signaling pathways by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. Activations of phosphatidylinositol 3-kinase (PI-3 kinase) and transcription factor NF-κB are involved in CT-1 mediated cellular processes. CT-1 has been implicated in the pathophysiology of certain heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure.

Human Cardiotrophin-1 ELISA Kit

 

All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

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