Human Cardiotrophin-1 ELISA Kit

Human Cardiotrophin-1 ELISA Kit

Assay Range

31.2-2,000 pg/mL  


10.0 pg/mL


No cross-reaction with other related substances detected




Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Detection Method




Kit Components


 1. Recombinant Human Cardiotrophin-1 standard: 2 vials

 2. One 96-well plate coated with Human Cardiotrophin-1  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1





Cardiotrophin1 (CT-1) is a member of the IL-6 family and is widely expressed in the heart, skeletal muscle, prostate, ovary, lung, kidney, pancreas, thymus, testis and small intestine with a higher level at heart and skeletal muscle. CT-1 is a cardiac hypertrophic factor with overlapping functions with other members of IL-6 family. Human and mouse CT-1 share 80% aa sequence identity and exhibit species cross-reactivity. CT-1 initiates its downstream signaling pathways by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. Activations of phosphatidylinositol 3-kinase (PI-3 kinase) and transcription factor NF-κB are involved in CT-1 mediated cellular processes. CT-1 has been implicated in the pathophysiology of certain heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure.

Human Cardiotrophin-1 ELISA Kit


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