Human TLR1 ELISA Kit

Human TLR1 ELISA Kit
FM-E100553
$359.00


Assay Range

156 - 10,000 pg/mL

Sensitivity

10.0 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples.

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Human TLR1 standard: 2 vials.

 2. One 96-well plate precoated with anti- Human TLR1 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100.

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

 

 

Background

 

Toll-like receptor 1 (TLR1), also known as Toll/interleukin-1 receptor-like protein (TIL) or CD281, is a member of the Toll-like receptor (TLR) family. The Toll like proteins are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. At least eleven mouse and ten human TLRs have been identified. TLRs are characteristic of a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain. The ECD of human TLR1 shares 20% -43% aa sequence identitity with human TLR2, 3, 4, 5 ,7, 8, 9 and 10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2. TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 cooperates with TLR2 in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols. Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization, activation of NFκB, cytokine secretion and the inflammatory response.

 

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