Human C3 Protein

Background:

Native human C3 is a naturally glycosylated (approximately 2.7%) polypeptide containing two disulfide-linked chains. C3 is essential to the activation of all three pathways of complement activation. Initiation of each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and activating C3b.

Storage:

-70℃ or below. Avoid freeze/thaw.

Source:

Normal human serum

Physical Appearance:

Frozen liquid

Purity:

>95% by SDS-PAGE

Molecular Weight:

Size:

185,000 Da (2 chains)

250 µg/vial

Concentration:

1.0 mg/ml

Activity:

>70% versus normal human serum standard  

Buffer:

10 mM sodium phosphate, 145 mM NaCl, pH 7.2

Preservative:

None, 0.22 µm filtered.