Human C5b,6 Complex

Background:

C5b, 6 is a product of complement activation of C5 and an intermediate in the formation of the C5b-9 membrane attack complex (MAC) of complement. Normally, the C5b, 6 complex remains bound to the C3/C5 convertase until it binds a single C7 molecule.  However, in the absence of the C7 protein, C5b, 6 is released into the fluid phase. C5b, 6 is a very stable complex. During complement activation, some C5b, 6 diffuses away from the target cell and may, after combining with C7, enter the membrane of a nearby cell. This is called bystander lysis or “reactive lysis” and can be a significant source of pathology. 

Storage:

-70℃ or below. Avoid freeze/thaw

Source:

Normal human serum

Activity:

Purity:

>10,000 Units/mg for lysis of sheep erythrocytes 

>90% by SDS-PAGE

Molecular Weight:

Size:

285,000 Da (3 chains)

50 µg/vial

Concentration:

0.2 mg/mL

Buffer:

10 mM HEPES, 120 mM NaCl, pH 7.2

Preservative:

None, 0.22 µm filtered