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Assay Range | 625 - 40,000 pg/mL |
Sensitivity | 600 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 50 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C8 standard: 1 vial.
2. One 96-well plate precoated with anti- Human C8 Ab
3. Diluent buffer (10x): 30 mL x1
4. Detection antibody: 1 vial
5. Streptavidin-HRP (100x): 80 µL x 1
6. TMB color developing agent : 8 mL x1.
7. TMB stop solution: 12 mL x1.
8. Washing solution (20x): 30 mL x2.
Background
Human C8 is a three-chain glycosylated protein with a total molecular weight of 151 KDa. The alpha chain (64 kDa) and the gamma chain (22 kDa) are disulfide linked. The beta chain (64,kDa) is non-covalently bound to the α/γ complex. C8α and C8β are highly homologous to each other and to C6, C7 and C9, and contain a common membrane attack complex/perforin (MACPF) domain. C8γ has a lipocalin fold and shares no homology with any other complement protein. C8 plays a key role in membrane attack complex MAC assembly by coordinating the interaction with complement proteins C5b-7 and the pore-forming protein C9 on pathogen membranes. It is also the first component to penetrate the lipid bilayer. C8 deficiency exhibits an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease.