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Human MMP-12 ELISA Kit

FM-E100799

$509.00

More info

Assay Range

62.5--4000 pg/mL

Sensitivity

10.0 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Human MMP-12 standard: 2 vials

 2. One 96-well plate precoated with anti- Human MMP-12 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

 

 

Background

 

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases which can degrade the extracellular matrix. Matrix metalloproteinase-12 (MMP-12), also known as macrophage elastase (ME), is a member of the MMP family and is encoded by the MMP-12 gene in humans. Pro-MMP-12 is composed of a pro-domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. Expression of MMP-12 in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling. In addition to elastin, MMP-12 has a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, α1antitrypsin, and plasminogen. MMP-12 is required for macrophage-mediated proteolysis and matrix invasion in mice. MMP-12 may play a role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. In addition, MMP-12 can also activate MMP-2 and MMP-3.

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