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Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human FGF4 standard: 2 vials
2. One 96-well plate precoated with anti-Human FGF4 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
FGF4 (fibroblast growth factor 4), also known as heparin secretory-transforming protein 1 (HST, HST-1, HSTF-1), Heparin-binding growth factor 4 (HBGF-4), FGFK or KFGF (Kaposi’s sarcoma associated FGF), is a secreted, heparin binding protein belonging to the FGF family. The full-length human FGF4 precursor is a 206 amino acid (aa) precursor protein consisting of a 33 aa signal peptide and a 173 aa mature protein with a heparin binding region near the C-terminus. Mature human FGF4 protein is 91% and 82% identical to mouse and rat FGF4, respectively. Expression of FGF4 is spatially and temporally regulated during embryonic development. FGF4 plays an important role in the regulation of embryonic development, cell proliferation and differentiation. FGF4 can bind to different members of the FGF receptor family including FGFR1, FGFR2, FGFR3 and FGFR4, to activate a variety of cellular processes involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.