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Assay Range | 62.5--4000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse sL-Selectin standard: 2 vials
2. One 96-well plate precoated with anti-Mouse sL-Selectin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
L-Selectin, also known as leukocyte selectin, Leukocyte adhesion molecule 1 (LAM-1), Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1) CD62L, LAM-1, LECCAM-1, TQ1, Leu-8, DREG, lymph node homing receptor, MEL-14 antigen, is a cell surface glycoprotein belonging to the selectin family. L-Selectin is characterized by an NH2-terminal calcium-dependent lectin domain, an EGF-like domain, two CRP-like domains, a transmembrane sequence and a short cytoplasmic domain. Human and mouse L-Selectin show the same domain structure and are 77% amino acid sequence identical.
L-selectin is an adhesion/homing receptor that plays important roles in lymphocyte-endothelial cell interactions. Three ligands (GlyCAM-1, sgp90/CD34, MAdCAM-1) for L-Selectin have been identified on endothelial cells, all containing O-glycosylated mucin or mucin-like domains. Two forms of soluble L-Selectin (sL-Selectin) with different molecular weight have been identified. The lymphocyte form is about 62 kDa, while the fragment derived from neutrophils is 75-100 kDa. sL-Selectin retains bioactivity, and at high concentrations can inhibit binding of lymphocytes to endothelium.