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Uracil DNA Glycosilase ( UNG )
DescriptionE.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).SourceEscherichia
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol. |
| Description | E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). |
| Expression host | Escherichia Coli strain that carries the UNG gene from E.coli. |
| Synonyms | Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles. |
| Unit Definition | 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. |
| Specific Activity | The Specific Activity was found to be 5U/ul. |
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Protein content | By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |