PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.
Greater than 90% as determined by SDS-PAGE.
Threonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton. PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.
Sf9 insect cells.
Threonyl-tRNA synthetase cytoplasmic, EC 188.8.131.52, Threonine-tRNA ligase, ThrRS, MGC9344, PL-7, TARS.
Sterile Filtered clear solution.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.