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Taq Plus DNA Polymerase Recombinant ( Taq Plus DNA )
DescriptionTaq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106
Data sheet
| Description | Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C. |
| Expression host | Recombinant E.coli contains Thermus aquaticus polymerase gene. |
| Synonyms | Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA. |
| Unit Definition | One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. |
| Storage Conditions | Stable for 5 days at 10°C, for longer period of time store at -20°C. |