In conventional ELISA protocol, coating of capture antibodies on ELISA plates takes the maximum time. To reduce the time involved in coating procedure, we have developed a fast coating buffer with comparable efficiency to regular coating buffer (PBS/ sodium bicarbonate). Efficient coating of capture antibodies on ELISA plates is achieved in 30 min. Capture antibody stability and kit performance is not compromised up on storage at 2-8 oC for up to 3 months.
Fig. 1: ELISA plates were coated with mouse anti-human relaxin-2 antibody using conventional coating buffer (overnight) or NovateinBio’s Fast Coating Buffer (30 min). Incubation time and temperature of remaining steps was not altered.
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