Trypsin-2 Human Recombinant

Trypsin-2 Human Recombinant
$189.00

Trypsin-2 Human Recombinant

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DescriptionRecombinant Human Trypsin-2 is free from any animal and human sources. Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques. Recombinant Human Trypsin-2 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin-2 is free from protease inhibitors such as PMSF and EDTA.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered lyophilized powder.
FormulationThe protein was lyophilized with 10mM Sodium Acetate buffer and 50mM NaCl.
SolubilityIt is recommended to reconstitute the lyophilized Human Trypsin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityRecombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 90% as determined by SDS-PAGE.
Unit DefinitionOne BAEE unit will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25C (1cm light path). Trypsin digestion: suggested ratio (w:w) 1:20 to 1:1000 (Trypsin : Peptide).
Biological Activity10,000 BAEE units/mg powder.

 

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