dCas9 (D10A & H840A) with NLS

dCas9 (D10A & H840A) with NLS
$275.00

dCas9 (D10A & H840A) with NLS

More details

Background
NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide- RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two- gRNA guided cleavage can significantly reduce the off target effects.
Purity
Greater than 90% as determined by SDS-PAGE.storage buffer
Host/Source
E.coli
Concentration
40 ug/ 40 ul, 250 pmol
storage buffer
10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage and Stability
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.


Nuclease deficient Cas9: Possess no cleavage activity due to D10A and H840A mutations. 
Has the ability to bind to DNA.

For Research Use Only

 

All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Contact us

Office hours: Mon-Fri 9am-5pm

Tel: (617) 238-1396
Fax: (617) 380-0053

Cart

No products

Shipping $0.00
Tax $0.00
Total $0.00

Cart