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Aflatoxin B1 ELISA kit
NR-R10184
Aflatoxin B1 competitive ELISA kit.
For research use only. Not for use in diagnostic procedures.
Data sheet
| Size | 96T |
| Category | Food ELISA kit |
| Background | Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions. Aflatoxins are the strongest mycotoxins, which act primarily in a hepatotoxic and carcinogenic way. The four main aflatoxins show a different toxicity. Aflatoxin M1 was the first metabolite of Aflatoxin B1, which could unequivocally be detected by Allcroft and Carnaghan in the milk of cows in 1963. Thus this first derivative was called Aflatoxin M1. As further investigations showed, also other mammalians excrete Aflatoxin M1 in milk, feces and urine. Contaminations of milk and milk products can be hazardous for human beings, because M1 is similar to Aflatoxin B1 regarding its hepatotoxicity. |
| Assay principle | The Aflatoxin M1 Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An aflatoxin conjugate is bound on the surface of a microtiter plate on the Aflatoxin M1 ELISA Kit. Aflatoxin M1 containing samples or standards and an antibody directed against aflatoxin M1 are given into the wells of the microtiter plate. |
| Assay Time | 2.5 hours |
| Assay Range | Competitive ELISA |
| Sensitivity | Colorimetric |
| Specificity | 10 pg/mL |
More info
The kit is used to quantify Aflatoxin B1 in food. This assay has high sensitivity and excellent specificity for detection of Aflatoxin B1. No significant cross-reactivity or interference between Aflatoxin B1 and analogues was observed.
Materials Provided with Aflatoxin M1 Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with aflatoxin conjugate
2. Aflatoxin M1 Standards (0; 100; 500; 1000; 5000; 10000 pg/mL): 6 vials with 0.5 mL
3. Anti-Aflatoxin M1 Antibody (rabbit): 6 mL
4. Conjugate (anti-rabbit-IgG-HRP): 15 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample/Standard Diluent (PBS): 60 mL.
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual
Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength
For research use only. Not for use in diagnostic procedures.