Lyophilized from 1mg/ml solution in glycine 0.05M buffer at pH 8.5 and 100mM NaCl.
It is recommended to reconstitute the lyophilized Myostatin in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Greater than 97.0% as determined by Analysis by SDS-PAGE.
Myostatin Human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 115 amino acids (molecular formula C586H865N165O164S12) and 6-His-tag at the N-terminal having the total molecular mass of 13.2kDa.
GDF8 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.
Nicotiana benthamiana plant
GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Myostatin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino acid sequence
HHHHHHDFGL DCDEHSTESR CCRYPLTVDF EAFGWDWIIA PKRYKANYCS GECEFVFLQKYPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII YGKIPAMVVD RCGCS