Cas9 (D10A) Nickase with NLS

Cas9 (D10A) Nickase with NLS
PR-137212B
$275.00
Cas9 (D10A) Nickase with NLS

More details

Storage
-20°C. Avoid freeze thaw cycles.
Background
NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide- RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two- gRNA guided cleavage can significantly reduce the off target effects.
Host/Source
E.coli
Concentration
40 ug/ 40 ul, 250 pmol
storage buffer
10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.


Cas9 D10A: Possesses only nickase activity.Cleaves only one DNA strand, and does not activate NHEJ

 
For Research Use Only

 

All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Contact us

Office hours: Mon-Fri 9am-5pm

Tel: (617) 238-1396
Fax: (617) 380-0053

Cart

No products

Shipping $0.00
Tax $0.00
Total $0.00

Cart