Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Biotinylated antibodies are designed to be detected using streptavidin or anti-biotin antibody conjugates.
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to Cas9 from Streptococcus pyogene.
This Cas9 antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cas9 Mouse mAb.
Dilution & Recommended Usage
Western blotting 1:1000, ELISA: 1:2000 to 1:8000
The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. The CRISPR antiviral mechanism of action involves three steps: (i), acquisition of foreign DNA by host bacterium; (ii), synthesis and maturation of CRISPR RNA (crRNA) followed by the formation of RNA-Cas nuclease protein complexes; and (iii), target interference through recognition of foreign DNA by the complex and its cleavage by Cas nuclease activity. The type II CRISPR/Cas antiviral immunity system provides a powerful tool for precise genome editing and has potential for specific gene regulation and therapeutic applications. The Cas9 protein and a guide RNA consisting of a fusion between a crRNA and a transactivating crRNA (tracrRNA) must be introduced or expressed in a cell. A 20-nucleotide sequence at the 5’ end of the guide RNA directs Cas9 to a specific DNA target site. As a result, Cas9 can be programmed to cut various DNA sites both in vitro and in cells and organisms. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells. Research studies demonstrate that CRISPR can be used to generate mutant alleles or reporter genes in rodents and primate embryonic stem cells. Specificity/Sensitivity: Cas9 (Cat#Cas9-mAb-bio) Mouse mAb (Biotinylated) recognizes transfected levels of total Cas9 protein. Source/Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogene.
Cas9 (7A9-3A3) Mouse mAb (Biotinylated) recognizes transfected levels of total Cas9 protein.