NLS-dCas9-EGFP-NLS

NLS-dCas9-EGFP-NLS
PR-137213G
$350.00
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Size
50 ug
Storage
-80°C. Avoid freeze thaw cycles.
Application
In situ hybridization, subcellular localization of defined sequence, enrich cell populations for desired genome edits via EGFP-based fluorescence activated cell sorting (FACS).
Background
NLS-dCas9-EGFP is a fusion protein containing a nuclear localization sequence (NLS) on its N terminal and EGFP on the C terminal. The dCas9 RNP complex can localize to the nucleus immediately upon entering the cell with the addition of a nuclear localization signal (NLS). The EGFP can be taken as a reporter for tracking or sorting transfected cells, which creates the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (FACS). NLS-dCas9-EGFP is better than NLS-Cas9-EGFP in genome tracking by in situ hybridization, due to the inactivity of nuclease while retaining the DNA binding ability
Purity
Greater than 90% as determined by SDS-PAGE
Host/Source
E.coli
Concentration
25 ug/ 25 ul
storage buffer
10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol
NLS-dCas9-EGFP-NLS

 

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