No products
View larger
Lipoteichoic acids, LTA ELISA Kit
Lipoteichoic acids,LTA ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Background | Lipoteichoic acid (LTA) is a major constituent of the cell wall of gram-positive bacteria. These organisms have an inner (or cytoplasmic) membrane and, external to it, a thick (up to 80 nanometer) peptidoglycan layer. The structure of LTA varies between the different species of Gram-positive bacteria and may contain long chains of ribitol or glycerol phosphate. LTA is anchored to the cell membrane via a diacylglycerol. It acts as regulator of autolytic wall enzymes (muramidases). It has antigenic properties being able to stimulate specific immune response. LTA may bind to target cells non-specifically through membrane phospholipids, or specifically to CD14 and to Toll-like receptors. Binding to TLR-2 has shown to induce NF-κB expression(a central transcription factor), elevating expression of both pro- and anti-apoptotic genes. Its activation also induces mitogen-activated protein kinases (MAPK) activation along with phosphoinositide 3-kinase activation. LTA's molecular structure has been found to have the strongest hydrophobic bonds of an entire bacteria. Said et al. showed that LTA causes an IL-10-dependent inhibition of CD4 T-cell expansion and function by up-regulating PD-1 levels on monocytes which leads to IL-10 production by monocytes after binding of PD-1 by PD-L. |
| Assay Type | Sandwich ELISA |
More info
|
Assay Range |
156-10,000 pg/mL |
|
Sensitivity |
75 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 - 8 ºC. |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Detection Method |
Chromogenic |
Structure:
Kit Components
1. Standard: 0.5 mL x 6 vials
2. One 96-well plate coated with capture Ab
3. Sample diluent buffer: 6 ml
4. HRP conjugate reagent: 10 ml
5. Chromogen solution A: 6 ml
6. Chromogen solution B: 6 ml
7. Stop solution: 6 mL x1
8. Washing solution (20x): 25 mL x1
9. Adhesive plate sealers: 2
10. Package insert: 1