Human High Mobility Group Box1 Protein HMGB1 ELISA Kit

Human High Mobility Group Box1 Protein HMGB1 ELISA Kit
BG-HUM11133
$499.50
Human High Mobility Group Box1 Protein ELISA Kit

More details

Assay Range
3.12 - 200 ng/ml
Storage
2-8 °C
Expiration
6 months
Assay principle
The Human HMGB1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse anti-Human HMGB1 specific antibody has been pre-coated onto 96-well plate. The test samples and the biotinylated goat anti-Human HMGB1 specific detection antibody are added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP is added and unbound conjugates are washed away with Wash Buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic Stop Solution. The density of yellow is proportional to the amount of Human HMGB1 captured on plate.


 

PRODUCT CITATIONS

S100B promotes glioma growth through chemoattraction of myeloid-derived macrophages

Wang H, Zhang L, Zhang IY, Chen X, Da Fonseca A, Wu S, Ren H, Badie S, Sadeghi S, Ouyang M, Warden CD, Badie B

Clin Cancer Res. 2013 Jul 15;19(14):3764-75

Sample: Cell culture supernatant

Human High Mobility Group Box1 Protein HMGB1 ELISA Kit

 

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