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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor protein p53 binding protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TP53BP1 |
Protein | Tumor suppressor p53-binding protein 1 |
Uniprot ID | Q12888 |
Function | Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Chromosome, centromere, kinetochore. Note: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed. |
Sequence Similarities | Contains 2 BRCT domains. |
Aliases | 53 BP1 antibody|53BP1 antibody|FLJ41424 antibody|MGC138366 antibody|p202 antibody|p53 binding protein 1 antibody|p53 BP1 antibody|p53-binding protein 1 antibody|p53BP1 antibody|TP53 BP1 antibody|TP53B_HUMAN antibody|TP53BP1 antibody|TRP53 BP1 antibody|Tumor protein 53 binding protein 1 antibody|Tumor protein p53 binding protein 1 antibody|Tumor suppressor p53 binding protein 1 antibody|Tumor suppressor p53-binding protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-53BP1 antibody, ASA-B0004, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: U20S Cell Lysate
Lane 3: HEPA Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: U20S Cell Lysate
Lane 3: HEPA Cell Lysate
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Human Intestinal Cancer Tissue
Human Intestinal Cancer Tissue
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Rat Brain Tissue