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Human Cryptosporidium Fecal ELISA Kit
NR-R10180
Human Cryptosporidium Fecal  ELISA KitÂ
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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSESÂ
Data sheet
| Background | Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men. Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. However, this method relies on an experienced technician and subsequent observation of intact organisms. One important alternative has been the development of an antigen capture ELISA for use with stools with comparable sensitivity to experienced microscopic examinations. |
| Assay principle | During the first incubation, Cryptosporidium specific antigen present in the stool specimens are captured by antibodies attached to the microwells. The wells are incubated and washed before anti Cryptosporidium antibodies conjugated to horseradish peroxidase are added. The enzyme conjugate will sandwich any antigen bound to the wells. After washings to remove unbound enzyme, a chromogen is added which develops a blue color in the presence of the enzyme complex. The stop solution ends the reaction and turns the blue color to yellow. |
More info
This kit is used to qualitatively detect Cryptosporidium Antigens in Human stool. Manual is updated. Please contact us at info@novateinbio.com for the newest manual.
| Full name |
Human Cryptosporidium Fecal ELISA Test Kit |
| Category Name | Parasitology ELISA kits |
|---|---|
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA principle- Sandwhich; Antibody Coated Plate |
| Detection Range | Qualitative - Positive, Negative Controls |
| Sample | 1gm Stool Sample |
| Specificity | 100% |
| Sensitivity | 100% |
| Total Time | ~100min |
| Shelf Life | 18 months from the manufacturing date |
Material Provided with Chagas ELISA kit:
1. Test Strips Microwells: 96 test wells anti-Cryptosporidium polyclonal antibodies
2. Enzyme Conjugate: anti-Cryptosporidium antibodies conjugated-HRP
3. Positive Control: diluted Cryptosporidium positive formalinized stool supernatant
4. Negative Control: diluted Cryptosporidium negative formalinized stool supernatant
5. TMB Substrate Solution: TMB and peroxid
6. Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution containing Thimerosal
8. Stop Solution: 5% phosphoric acid