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Assay Range |
78.1 - 5,000 pg/mL |
|
Sensitivity |
10.0 pg/mL |
|
Size |
96T |
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Storage |
Store at 2 - 8ºC. |
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Assay Principle |
Sandwich ELISA |
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Sample volume |
100 µL final volume, dilution factor varies on samples. |
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Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human NGAL standard: 2 vials.
2. One 96-well plate precoated with anti- Human NGAL Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Lipocalin-2, also known as Neutrophil Gelatinase-associated Lipocalin (NGAL), 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25, or Siderocalin, is a member of the Lipocalin family which functions as transporters for small hydrophobic molecules such as steroid hormones, vitamins, odorants, and metabolic products. Lipocalin-2 exists in monomer, homodimer, and heterodimer which is associated with human matrix metalloproteinase 9 (MMP-9). The Lipocalin-2/MMP-9 complex may modulate protease activity by protecting MMP-9 from degradation. Human Lipocalin-2 shares 62% amino acid (aa) sequence identity with mouse ortholog (also known as 24p3).
It is shown that Lipocalin-2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Lipocalin-2 binds iron by association with 2, 5-dihydroxybenzoic acid (2, 5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell in a context- dependent manner. Following binding to the SLC22A17 (24p3R) receptor, Iron-bound form of Lipocalin-2 is internalized to release iron and increase intracellular iron concentration. In contrast, interaction of the iron-free form with 24p3R receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Notably, iron-free form decreases intracellular iron levels and induces expression of the proapoptotic protein BCL2L11/BIM to initiate apoptosis.