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Vitamin B12,VB12 ELISA Kit

NR-R10091

$756.00

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Principle
Competitive ELISA
 
Detection Range
0 - 2000 pg/mL
 
Size
96T
 
Sensitivity                      70 pg/mL
 
Assay time
~ 120 min
 
Shelf Life
12~14 Months from the Manufacturing Date
 
Storage
Store at 2 - 8ºC 
 
Sample 
Serum, Plasma
 
Detection Method
Chromogenic
  
Kit Components
 
 
 
1. standard Set: 6 vials
 
 
2. One 96-well plate precoated with antibody
 
 
3. Biotinated VB12 specific antibody: 1 vial
 
 
4. HRP conjugated VB12: 1 vial
 
 
5. 25 x wash buffer:1bottle
 
 
6. TMB substrate: 1 vial
 
 
7. TMB stop solution: 12 ml.

 

Background

 

Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood.. Vitamin B12 was discovered from its relationship to disease pernicious anemia, which is an autoimmune disease in which parietal cells of the stomach responsible for secreting intrinsic factor are destroyed (these cells are also responsible for secreting acid in the stomach). It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid metabolism and amino acid metabolism.  Vitamin B12 consists of a class of chemically related compounds (vitamers), all of which have vitamin activity. 

standard Curve

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