Mouse Interleukin 2,IL-2 ELISA Kit

Mouse Interleukin 2,IL-2 ELISA Kit
$539.00



Assay Range

15.6--1000 pg/mL  

Sensitivity

1.0 pg/mL

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Sample Type

serum, plasma or cell culture supernatant 

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Mouse IL-2 standard: 2 vials

 2. One 96-well plate coated with Mouse IL-2  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

 9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1

 

 

Background

 

Human Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a member of the common gamma chain (γc) cytokine family. The 153 amino acid (aa) human IL-2 precursor contains a 20 aa signal sequence and a 133 aa mature region with one O-linked glycosylation site at Thr3, plus three cysteines, two of which form an intrachain disulfide bond that is essential for IL-2 bioactivity. Mouse IL-2 cDNA encodes a 169 amino acid (aa) precursor containing a 20 aa signal sequence and a 149 aa mature chain. Human IL-2 shares approximately 60% sequence identity with mouse IL-2. To some extent, mouse and human IL-2 exhibit species cross-reactivity. IL-2 is expressed and secreted on γδT cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells in humans.

IL-2 first binds to IL-2 receptor α (IL-2Rα)/ CD25 in the binary receptor complex then recruits IL-2Rβ/CD122 and IL-2Rγ/CD132 to trigger a variety of biological processes. IL-2 plays a key role in the development, maintenance and function of regulatory T cells and in promoting the development of activated CD8+ T cells into memory cells.

Mouse Interleukin 2,IL-2 ELISA Kit

 

All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

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