No cross-reaction with other related substances detected
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC
100 µL final volume, dilution factor varies on samples
serum, plasma or cell culture supernatant
1. Recombinant Mouse IL-2 standard: 2 vials
2. One 96-well plate coated with Mouse IL-2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Human Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a member of the common gamma chain (Î³c) cytokine family. The 153 amino acid (aa) human IL-2 precursor contains a 20 aa signal sequence and a 133 aa mature region with one O-linked glycosylation site at Thr3, plus three cysteines, two of which form an intrachain disulfide bond that is essential for IL-2 bioactivity. Mouse IL-2 cDNA encodes a 169 amino acid (aa) precursor containing a 20 aa signal sequence and a 149 aa mature chain. Human IL-2 shares approximately 60% sequence identity with mouse IL-2. To some extent, mouse and human IL-2 exhibit species cross-reactivity. IL-2 is expressed and secreted on Î³Î´T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells in humans.
IL-2 first binds to IL-2 receptor Î± (IL-2RÎ±)/ CD25 in the binary receptor complex then recruits IL-2Rβ/CD122 and IL-2RÎ³/CD132 to trigger a variety of biological processes. IL-2 plays a key role in the development, maintenance and function of regulatory T cells and in promoting the development of activated CD8+ T cells into memory cells.
All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Office hours: Mon-Fri 9am-5pm
Tel: (617) 238-1396
Fax: (617) 380-0053
NovaTeinBio is a one-stop solution for antibody production and purification services
As a leading manufacturer and... view More