Mouse Leptin receptor,LR/Ob-R ELISA Kit

Mouse Leptin receptor,LR/Ob-R ELISA Kit
$539.00



Assay Range

156 - 10,000 pg/mL

Sensitivity

5.0 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Mouse Leptin receptor standard: 2 vials

 2. One 96-well plate precoated with anti- Mouse Leptin receptor Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

 

 

Background

 

Leptin receptor (Leptin R), also known as B219, OB receptor (OB-R), or CD295, is a 150 kDa protein belonging to the class I cytokine receptor family. Mouse Leptin R is synthesized as a 1162 amino acid (aa) polypeptide composed of a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain linked to JAK/STAT pathway. Mouse Leptin R shares approximately 78% and 93% aa sequence identity with human and rat Leptin R, respectively. Several alternative spliced forms (a-f) of obR gene have been identified. ObRa, ObRc, ObRd and ObRf with truncated cytoplasmic domains, are responsible for mediating Leptin binding and endocytosis, but not signal transduction, while ObRb (formerly OB RL) containing a large cytoplasmic domains, expressed mainly in the hypothalamic arcuate nucleus, is essential for signal transduction. Mutations of ObRb can cause obese phenotypes in both the mouse and rat. In addition, a soluble Leptin R has been identified and is the primary Leptin-binding protein in blood to maintain a pool of available bioactive Leptin, delay Leptin clearance from circulation, and down-regulate blood brain transmission of Leptin.

 

All of Novateinbio's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to Novateinbio that you will properly test and use any Products purchased from Novateinbio in accordance with industry standards. Novateinbio and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

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