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Assay Range | 31.2-2,000 pg/mL |
Sensitivity | 3.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat TIMP-1 standard: 2 vials
2. One 96-well plate coated with Rat TIMP-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
The tissue inhibitor of metalloproteinase 1 (TIMP-1), also known as erythroid- potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor), is a member of the tissue inhibitor of metalloproteinase (TIMP) family in which four proteins TIMP-1, TIMP-2, TIMP-3 and TIMP-4 have been identified so far. TIMPs are natural inhibitors of the matrix metalloproteinases (MMPs) which are involved in degradation of the extracellular matrix and exhibit diverse functions in development, tissue remodeling, wound healing and tumor metastasis. TIMPs block access of substrates to the MMP catalytic site by forming 1:1 non-covalent complexes with MMPs. Basically, TIMPs are highly specific for MMPs but may not be able to digest some particular MMP such as MMP-14.
The full-length rat TIMP-1 is a 217 amino acid (aa) secreted glycoprotein composed of a 23 aa signal peptide and a 194 aa mature form. Rat TIMP-1 shares 85% and 70% aa sequence identity with mouse and human TIMP-1, respectively. Among the three known rat TIMPs, TIMP-1 shares 40% and 35% aa identity with TIMP-2 and TIMP-3, respectively. TIMP-1 is widely expressed by many cells and tissues, and is inducible in response to inflammatory stimuli of IL-1, IL-6, OSM, LIF, TNF-α, TGF-β1 and phorbol esters. In addition to its inhibitory activity on MMPs, TIMP-1 also functions as a growth factor that regulates cell differentiation, migration and death and shows erythroid potentiating activity.