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Haptoglobin Human Recombinant ( Haptoglobin Human )
DescriptionHaptoglobin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing (aa. 145-405) fusion protein with His tag and having a total Mw of 33 kDa (4 kDa His-tag).SourceEscherichia Coli.Physical
Data sheet
| Formulation | Lyophilized with no additives. |
| Solubility | It is recommended to reconstitute the lyophilized Haptoglobin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0%. |
| Applications | 1. Positive control for Western blot 2. Antibody production 3. Protein assay. |
| Description | Human Haptoglobin produced from pooled human plasma corresponding to the Mw of isotypes of Haptoglobin at 86kD and >200kD. |
| Protein Background | Haptoglobin is a glycoprotein which is synthesized in the liver and circulates in the blood. Haptoglobin is produced typically by hepatocytes but also by other tissues: e.g. skin, lung, and kidney. It is a positive acute phase protein that binds free hemoglobin and removes it from the circulation to prevent kidney injury, and iron loss following hemolysis. The haptoglobin-hemoglobin complex is subsequently removed by the reticuloendothelial system (generally the spleen). As the reticuloendothelial system removes the haptoglobin-hemoglobin complex from the body, haptoglobin levels are reduced in hemolytic anaemias. In the course of binding hemoglobin, haptoglobin sequesters the iron inside hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. Haptoglobin consists of two a- and two b-chains, connected by disulfide bonds. Three major haptoglobin phenotypes are known to exist (Hp 1-1, Hp 2-1, and Hp 2-2). Hp 1-1 is biologically the most effective in binding free hemoglobin and suppressing inflammatory responses associated with free hemoglobin. Hp 2-2 is biologically the least active, and Hp 2-1 is moderately active. Haptoglobins molecular mass ranges from 8-200 kDa.Reduced levels can be seen in haemolysis and impaired liver function. High levels are a marker for acute or chronic inflammation. Ahaptoglobinemia or hypohaptoglobinemia are caused by mutations in the haptoglobin gene and/or its regulatory regions. Haptoglobin is also linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. |
| Expression host | Pooled human plasma. |
| Synonyms | Haptoglobin, HP, BP, HPA1S, MGC111141, HP2-ALPHA-2. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Human Haptoglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL18 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Product Description | Immunoreactive with all sera of HIV-1 infected individuals. |