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Centromere Protein-H Human Recombinant ( CENPH Human )

DescriptionCENPH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (136-247) and having a molecular mass of 15.5 kDa.The CENPH is fused to a 20 amino acid His-Tag at N-terminus and

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Data sheet

Formulation The CENPH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Purity Greater than 90% as determined by SDS-PAGE.
Description CENPH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (136-247) and having a molecular mass of 15.5 kDa.The CENPH is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Protein Background CENPH is a member of the centromere protein H family. CENPH protein is a component of the CENPA-NAC (nucleosome-associated) complex which has a vital part in assembly of kinetochore proteins. The CENPA-NAC complex utilizes the CENPA-CAD (nucleosome distal) complex and is involved in integration of freshly synthesized CENPA into centromeres.
Expression host E.coli.
Synonyms Centromere protein H, Interphase centromere complex protein 35, CENP-H, NNF1, PMF1, ICEN35, Kinetochore protein CENP-H.
Reagent Appearance Sterile Filtered colorless solution.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

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