> >>Ras Homolog Gene Family Member A Human Recombinant ( RHOA Human )
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Ras Homolog Gene Family Member A Human Recombinant ( RHOA Human )

$193.00

Data sheet

Formulation The RHOA solution (1 mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Purity Greater than 90.0% as determined by SDS-PAGE.
Description RHOA Human Recombinant fused with a 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 227 amino acids (1-190 a.a.) and having a molecular mass of 25.7kDa (Molecular size on SDS-PAGE will appear higher). The RHOA is purified by proprietary chromatographic techniques.
Protein Background RhoA is a member of the small GTPase superfamily, Rho family, whose members are involved in the regulation and timing of cell division. RHOA is a small GTPase protein which acts to regulate the actin cytoskeleton in the formation of stress fibers and also upon 2 known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila).
Expression host Escherichia Coli.
Synonyms Transforming protein RhoA, Rho cDNA clone 12, h12, RHOA, ARH12, ARHA, RHO12, RHOH12.
Reagent Appearance Sterile Filtered colorless solution.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino acid sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC.

More info

ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

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