> >>Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant, Sf9 ( SNRPD3 Human, Sf9 )
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Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant, Sf9 ( SNRPD3 Human, Sf9 )

$193.00

Data sheet

Formulation SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Purity Greater than 80% as determined by SDS-PAGE.
Applications Western blot with anti-Sm positive patient sera.
Description SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton. SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Protein Background SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript variants encoding the same protein were branded.
Expression host Sf9 insect cells.
Synonyms Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.
Reagent Appearance Sterile Filtered clear solution.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Immunological functions 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Coating concentration 0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

More info

Sm-D3, snRNP core protein D3, Small nuclear ribonucleoprotein D3 polypeptide 18kDa

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