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Small Nuclear Ribonucleoprotein Polypeptide D1, D2, D3 Human Recombinant ( SNRPD Human )
Data sheet
| Formulation | SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity | Greater than 80% as determined by SDS-PAGE. |
| Applications | Western blot with:a) Anti-Sm positive patient sera.b) Monoclonal anti-hexa-His-tag antibody. |
| Description | SNRPD Human Recombinant produced in SF9 insect cells, is a mixed protein complex containing the SNRPD1 (14,306 Dalton), SNRPD2 (14,773 Dalton) and SNRPD3 (14,739 Dalton) proteins. Each protein (SNRPD1+ SNRPD2+ SNRPD3) is individually fused to a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
| Expression host | Sf9 insect cells. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels). |
| Coating concentration | 0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. |