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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TNF receptor-associated factor 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TRAF3(387-403aa RHDQMLSVHDIRLADMD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRAF3 |
Protein | TNF receptor-associated factor 3 |
Uniprot ID | Q13114 |
Function | Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm . Endosome . Mitochondrion. Note: Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus. |
Sequence Similarities | Belongs to the TNF receptor-associated factor family. A subfamily. |
Aliases | CAP 1 antibody|CAP-1 antibody|CAP1 antibody|CD40 associated protein 1 antibody|CD40 binding protein antibody|CD40 bp antibody|CD40 receptor associated factor 1 antibody|CD40 receptor-associated factor 1 antibody|CD40-binding protein antibody|CD40bp antibody|CRAF 1 antibody|CRAF1 antibody|IIAE5 antibody|LAP 1 antibody|LAP1 antibody|LMP1 associated protein antibody|LMP1-associated protein 1 antibody|MIPT3 antibody|TNF receptor associated factor 3 antibody|TNF receptor-associated factor 3 antibody|TRAF 3 antibody|Traf3 antibody|TRAF3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAF3 antibody, ASA-B1903, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3, 30.6KD(162aa tag+ R341-Y449)
Lane 1: Recombinant Human TRAF3 Protein 5ng
Lane 2: Recombinant Human TRAF3 Protein 2.5ng
Lane 3: Recombinant Human TRAF3 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3, 30.6KD(162aa tag+ R341-Y449)
Lane 1: Recombinant Human TRAF3 Protein 5ng
Lane 2: Recombinant Human TRAF3 Protein 2.5ng
Lane 3: Recombinant Human TRAF3 Protein 1.25ng
