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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Sp4 transcription factor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Transcription factor Sp4(29-44aa ENNNKKPKTSGSQDSQ), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SP4 |
Protein | Transcription factor Sp4 |
Uniprot ID | Q02446 |
Function | Binds to GT and GC boxes promoters elements. Probable transcriptional activator. |
Tissue Specificity | Abundant in brain. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the Sp1 C2H2-type zinc-finger protein family. |
Aliases | HF1B antibody|MGC130008 antibody|MGC130009 antibody|Sp4 antibody|SP4_HUMAN antibody|SPR-1 antibody|SPR1 antibody|Transcription factor Sp4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Transcription factor Sp4 antibody, ASA-B1912, Western blotting
All lanes: Anti Transcription factor Sp4 (ASA-B1912) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
All lanes: Anti Transcription factor Sp4 (ASA-B1912) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
