Characteristics of Streptococcus pyogenes Cas9 variants used for genome editing

 

Product nameCatalog numberCRISPR-Cas9 variantsAmino acid featuresNLS positionApplicationPAM sequenceAmountConcentrationPrice
Cas9 nucleasePR-137210Streptococcus pyogenesCas9 wild typewild typeN-terminalCreate double blunt end for recombinationNGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$105
NLS-Cas9-NLS PR-137211Streptococcus pyogenesCas9 wild typewild typeN-terminal and C-terminalCreate double blunt end for recombinationNGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$105
NLS-Cas9-EGFPPR-137211-EStreptococcus pyogenesCas9 with C-terminal EGFPwild typeN-terminal and C-terminalCreate double blunt end for recombinationNGG50   µg, 265 pmol1 µg/ µL, 5.31 µM$250
Cas9(D10A) nickasePR-137212Streptococcus pyogenesCas9 nickaseD10AN-terminalCreate nick on one strandNGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$195
NLS-Cas9(D10A)-2NLS nickasePR-137212BStreptococcus pyogenesCas9 nickaseD10AN-terminal and 2xC-terminalCreate nick on one strandNGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$195
dCas9 (D10A & H840A)PR-137213no nuclease activity while keeping target binding activityD10A/H840AC-terminalDetection and purification targeted DNANGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$250
NLS-dCas9-NLSPR-137213Bno nuclease activity while keeping target binding activityD10A/H840AN-terminal and C-terminalDetection and purification targeted DNANGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$250
Biotinylated NLS-dCas9-NLSPR-137213-biono nuclease activity while keeping target binding activity, biotinylatedD10A/H840AN-terminal and C-terminalSpecific DNA fragment pull-down in CHIP assay. FISH and short sequence dtectionNGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$250
NLS-dCas9-EGFP-NLSPR-137213Gno nuclease activity while keeping target binding activityD10A/H840AN-terminal and C-terminalFISH, genomic visualizationNGG50   µg, 265 pmol1 µg/ µL, 5.31 µM$245
eSpCas9(1.1)-3NLSCas9-HiFi1Cas9 with increase On-target specificityK848A/K1003A/R1060AN-terminal and 2xC-terminalgene editing with better on-target, less off-target rate.NGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$195
SpCas9-HF1-3NLSCas9-HiFi2Cas9 with increase On-target specificityN497A/R661A/Q695A/Q926AN-terminal and 2xC-terminalgene editing with better on-target, less off-target rate.NGG50   µg, 313 pmol1  µg/ µL, 6.26 µM$195
Cas9 proteinCAS9-OEM     Any size please inquire
Cas9 proteinCAS9-Trial     Small SIze Available please inquire



 

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is becoming an essential tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 is  designated to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing much easier. The nuclease-deactivated form of dCas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome in nucleus, as well as for rewriting the epigenetic dynamics, all in a sequence-specific manner. With all of these progress, scientists have begun to explore the possible applications of Cas9 in biomedical research and therapeutics. Novatein Biosciences is working hard to help you in fully utilize the Cas9 related proteins, antibodies, and assay kit to advance your projects. We focus on the applications of Cas9 proteins for genome editing, regulation, and subcellular localization of target genes.
 


A comprehensive Cas9 reagents ready to meet your research and development needs.
NLS-Cas9-NLS (Catalog number: PR-137211) transfection ready for gene editing, also used for sgRNA or crRNA screening (Catalog number: NB-GEN10642)

 

NLS-dCas9-NLS (Catalog number: PR-137213B) for precise gene regulation and interrogation.

 

NLS-Cas9-EGFP (Catalog number: PR-137211-E) for subcellular localization of the Cas9-gRNA/target DNA complex.

 

sgRNA Screening Kit (Catalog number: NB-GEN10642) for quickly assessing a sgRNA sequence in vitro to see if it can work effectively in vivo.

 

Anti-CRISPR/CAS9 Monoclonal Antibody (Catalog number: SH-A16960) detection of Cas9 ( wild type, nickase and dCas9) in WB, IF, and ELISA

 

Anti-CRISPR/CAS9 Polyclonal Antibody (Catalog number: SH-A16959) detection of Cas9 ( wild type, nickase and dCas9) in WB, IF, and ELISA

 

NLS-Cas9(D10A)-2NLS Nickase (Catalog number: PR-137212B) transfection ready for gene editing, single nick creation on double strand DNA.

 

References

 1.      Deactivated CRISPR Associated Protein 9 for Minor-Allele Enrichment in Cell-Free DNA    Amin Aalipour et al. Clinical Chemistry  2018 Vol. 64, Issue 2 p307-p916

2.       Purified Cas9 Fusion Proteins for Advanced Genome Manipulation Jovan Mircetic et al. Small Methods 2017 1, 1600052

3.       Disruptive non-disruptive applications of CRISPR/Cas9  Jonathan LSchmid-Burgk  Current Opinion in Biotechnology   December 2017, Volume 48 Pages 203-209

4.       Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins Toshitsugu Fujita et al. Genes to Cells Volume 21, Issue 4 April 2016  Pages 370–377

5.       High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding   Evan A Boyle  et al. PNAS    2017 May, 114 (21) 5461-5466

6.    CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells  Wulan Deng et al. PNAS 2015  vol. 112 | no. 38 p11870-11875

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